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Distinct expression patterns of HCN channels in HL-1 cardiomyocytes.

ABSTRACT:

Background

Cardiac rhythmic activity is initiated in functionally specialized areas of the heart. Hyperpolarization-activated and cyclic nucleotide-gated (HCN) channels are fundamental for these processes of cardiac physiology.

Results

Here we investigated transcript and protein expression patterns of HCN channels in HL-1 cardiomyocytes using a combination of quantitative PCR analysis and immunocytochemistry. Gene expression profiles of hcn1, hcn2 and hcn4 were acutely affected during HL-1 cell propagation. In addition, distinct expression patterns were uncovered for HCN1, HCN2 and HCN4 proteins.

Conclusions

Our results suggest that HCN channel isoforms might be involved in the concerted differentiation of HL-1 cells and may indirectly affect the occurrence of contractile HL-1 cell activity. We expect that these findings will promote studies on other molecular markers that contribute to cardiac physiology.

SUBMITTER: Gunther A

PROVIDER: S-EPMC4490601 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature

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Distinct expression patterns of HCN channels in HL-1 cardiomyocytes.

Günther Anne A Baumann Arnd A

BMC cell biology 20150704

<h4>Background</h4>Cardiac rhythmic activity is initiated in functionally specialized areas of the heart. Hyperpolarization-activated and cyclic nucleotide-gated (HCN) channels are fundamental for these processes of cardiac physiology.<h4>Results</h4>Here we investigated transcript and protein expression patterns of HCN channels in HL-1 cardiomyocytes using a combination of quantitative PCR analysis and immunocytochemistry. Gene expression profiles of hcn1, hcn2 and hcn4 were acutely affected du ...[more]

PMID: 26141616

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