Ontology highlight
ABSTRACT:
SUBMITTER: Hsu CH
PROVIDER: S-EPMC4493921 | biostudies-literature | 2015 Mar
REPOSITORIES: biostudies-literature
Hsu Chia-Hsien CH Chen Chihchen C Irimia Daniel D Toner Mehmet M
Technology 20150301 1
The isolation of CD4 positive T lymphocyte (CD4+) from peripheral blood is important for monitoring patients after HIV infection. Here, we demonstrate a fast isolation strategy for CD4+ cells that involves mixing blood and glass microbubbles. After the specific binding of target cells to the microbubbles carrying specific antibodies on their surface, target cells will spontaneously float to the top of the blood vial and can be quickly separated. Using this strategy, we demonstrate that the isola ...[more]