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Genetic Incorporation of ?-N-2-Hydroxyisobutyryl-lysine into Recombinant Histones.

ABSTRACT: Here, we report the evolution of an orthogonal amber suppressor pyrrolysyl-tRNA synthetase (PylRS)/tRNACUA(Pyl) pair that genetically encodes the post-translationally modified amino acid, ?-N-2-hydroxyisobutyryl-lysine (HibK), in bacteria and mammalian cells. HibK is a new type of histone mark that is widely distributed in histone proteins. The ability to site-specifically incorporate HibK into proteins provides a useful tool to probe the biological function of this newly identified post-translational modification.

SUBMITTER: Xiao H

PROVIDER: S-EPMC4506705 | biostudies-literature | 2015 Jul

REPOSITORIES: biostudies-literature

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Genetic Incorporation of ε-N-2-Hydroxyisobutyryl-lysine into Recombinant Histones.

Xiao Han H Xuan Weimin W Shao Sida S Liu Tao T Schultz Peter G PG

ACS chemical biology 20150429 7

Here, we report the evolution of an orthogonal amber suppressor pyrrolysyl-tRNA synthetase (PylRS)/tRNACUA(Pyl) pair that genetically encodes the post-translationally modified amino acid, ε-N-2-hydroxyisobutyryl-lysine (HibK), in bacteria and mammalian cells. HibK is a new type of histone mark that is widely distributed in histone proteins. The ability to site-specifically incorporate HibK into proteins provides a useful tool to probe the biological function of this newly identified post-transla ...[more]

PMID: 25909834

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