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Structural and Functional Characterization of the Hazelnut Allergen Cor a 8.

ABSTRACT: Nonspecific lipid transfer proteins (nsLTPs) are basic proteins, stabilized by four disulfide bonds, and are expressed throughout the plant kingdom. These proteins are also known as important allergens in fruits and tree nuts. In this study, the nsLTP from hazelnuts, Cor a 8, was purified and its crystal structure determined. The protein is stable at low pH and refolds after thermal denaturation. Molecular dynamics simulations were used to provide an insight into conformational changes of Cor a 8 upon ligand binding. When known epitope areas from Pru p 3 were compared to those of Cor a 8, differences were obvious, which may contribute to limited cross-reactivity between peach and hazelnut allergens. Differences in epitope regions may contribute to limited cross-reactivity between Cor a 8 and nsLTPs from other plant sources. The structure of Cor a 8 represents the first resolved structure of a hazelnut allergen.

SUBMITTER: Offermann LR 

PROVIDER: S-EPMC4616228 | biostudies-literature | 2015 Oct

REPOSITORIES: biostudies-literature

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Structural and Functional Characterization of the Hazelnut Allergen Cor a 8.

Offermann Lesa R LR   Bublin Merima M   Perdue Makenzie L ML   Pfeifer Sabine S   Dubiela Pawel P   Borowski Tomasz T   Chruszcz Maksymilian M   Hoffmann-Sommergruber Karin K  

Journal of agricultural and food chemistry 20151007 41

Nonspecific lipid transfer proteins (nsLTPs) are basic proteins, stabilized by four disulfide bonds, and are expressed throughout the plant kingdom. These proteins are also known as important allergens in fruits and tree nuts. In this study, the nsLTP from hazelnuts, Cor a 8, was purified and its crystal structure determined. The protein is stable at low pH and refolds after thermal denaturation. Molecular dynamics simulations were used to provide an insight into conformational changes of Cor a  ...[more]

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