Unknown

Dataset Information

0

Pulsed Azidohomoalanine Labeling in Mammals (PALM) Detects Changes in Liver-Specific LKB1 Knockout Mice.


ABSTRACT: Quantification of proteomes by mass spectrometry has proven to be useful to study human pathology recapitulated in cellular or animal models of disease. Enriching and quantifying newly synthesized proteins (NSPs) at set time points by mass spectrometry has the potential to identify important early regulatory or expression changes associated with disease states or perturbations. NSP can be enriched from proteomes by employing pulsed introduction of the noncanonical amino acid, azidohomoalanine (AHA). We demonstrate that pulsed introduction of AHA in the feed of mice can label and identify NSP from multiple tissues. Furthermore, we quantitate differences in new protein expression resulting from CRE-LOX initiated knockout of LKB1 in mouse livers. Overall, the PALM strategy allows for the first time in vivo labeling of mouse tissues to differentiate protein synthesis rates at discrete time points.

SUBMITTER: McClatchy DB 

PROVIDER: S-EPMC4642245 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature

altmetric image

Publications

Pulsed Azidohomoalanine Labeling in Mammals (PALM) Detects Changes in Liver-Specific LKB1 Knockout Mice.

McClatchy Daniel B DB   Ma Yuanhui Y   Liu Chao C   Stein Benjamin D BD   Martínez-Bartolomé Salvador S   Vasquez Debbie D   Hellberg Kristina K   Shaw Reuben J RJ   Yates John R JR  

Journal of proteome research 20151026 11


Quantification of proteomes by mass spectrometry has proven to be useful to study human pathology recapitulated in cellular or animal models of disease. Enriching and quantifying newly synthesized proteins (NSPs) at set time points by mass spectrometry has the potential to identify important early regulatory or expression changes associated with disease states or perturbations. NSP can be enriched from proteomes by employing pulsed introduction of the noncanonical amino acid, azidohomoalanine (A  ...[more]

Similar Datasets

| S-EPMC2886679 | biostudies-literature
| S-EPMC4558394 | biostudies-literature
| S-EPMC4215516 | biostudies-literature
2022-06-02 | PXD007749 | Pride
| S-EPMC7705018 | biostudies-literature
| S-EPMC8351166 | biostudies-literature
| S-EPMC2737075 | biostudies-literature
| S-EPMC7554132 | biostudies-literature
| S-EPMC1636784 | biostudies-literature
| S-EPMC5652135 | biostudies-literature