Dataset Information

Modulation of Human Macrophage Responses to Mycobacterium tuberculosis by Silver Nanoparticles of Different Size and Surface Modification.

ABSTRACT: Exposure to silver nanoparticles (AgNP) used in consumer products carries potential health risks including increased susceptibility to infectious pathogens. Systematic assessments of antimicrobial macrophage immune responses in the context of AgNP exposure are important because uptake of AgNP by macrophages may lead to alterations of innate immune cell functions. In this study we examined the effects of exposure to AgNP with different particle sizes (20 and 110 nm diameters) and surface chemistry (citrate or polyvinlypyrrolidone capping) on cellular toxicity and innate immune responses against Mycobacterium tuberculosis (M.tb) by human monocyte-derived macrophages (MDM). Exposures of MDM to AgNP significantly reduced cellular viability, increased IL8 and decreased IL10 mRNA expression. Exposure of M.tb-infected MDM to AgNP suppressed M.tb-induced expression of IL1B, IL10, and TNFA mRNA. Furthermore, M.tb-induced IL-1?, a cytokine critical for host resistance to M.tb, was inhibited by AgNP but not by carbon black particles indicating that the observed immunosuppressive effects of AgNP are particle specific. Suppressive effects of AgNP on the M.tb-induced host immune responses were in part due to AgNP-mediated interferences with the TLR signaling pathways that culminate in the activation of the transcription factor NF-?B. AgNP exposure suppressed M.tb-induced expression of a subset of NF-?B mediated genes (CSF2, CSF3, IFNG, IL1A, IL1B, IL6, IL10, TNFA, NFKB1A). In addition, AgNP exposure increased the expression of HSPA1A mRNA and the corresponding stress-induced Hsp72 protein. Up-regulation of Hsp72 by AgNP can suppress M.tb-induced NF-?B activation and host immune responses. The observed ability of AgNP to modulate infectious pathogen-induced immune responses has important public health implications.

SUBMITTER: Sarkar S

PROVIDER: S-EPMC4651328 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature

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Modulation of Human Macrophage Responses to Mycobacterium tuberculosis by Silver Nanoparticles of Different Size and Surface Modification.

Sarkar Srijata S Leo Bey Fen BF Carranza Claudia C Chen Shu S Rivas-Santiago Cesar C Porter Alexandra E AE Ryan Mary P MP Gow Andrew A Chung Kian Fan KF Tetley Teresa D TD Zhang Junfeng Jim JJ Georgopoulos Panos G PG Ohman-Strickland Pamela A PA Schwander Stephan S

PloS one 20151118 11

Exposure to silver nanoparticles (AgNP) used in consumer products carries potential health risks including increased susceptibility to infectious pathogens. Systematic assessments of antimicrobial macrophage immune responses in the context of AgNP exposure are important because uptake of AgNP by macrophages may lead to alterations of innate immune cell functions. In this study we examined the effects of exposure to AgNP with different particle sizes (20 and 110 nm diameters) and surface chemistr ...[more]

PMID: 26580078

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Project description:The growing use of silver nanoparticles (Ag-NPs) in consumer products raises concerns about their toxicological potential. The purpose of the study was to investigate the size- and coating-dependent pulmonary toxicity of Ag-NPs in vitro and in vivo, using an ovalbumin (OVA)-mouse allergy model. Supernatants from (5.6-45 µg/mL) Ag50-PVP, Ag200-PVP or Ag50-citrate-treated NR8383 alveolar macrophages were tested for lactate dehydrogenase and glucuronidase activity, tumor necrosis factor (TNF)-? release and reactive oxygen species (ROS) production. For the in vivo study, NPs were intratracheally instilled in non-sensitized (NS) and OVA-sensitized (S) mice (1-50 µg/mouse) prior to OVA-challenge and bronchoalveolar lavage fluid (BALF) inflammatory infiltrate was evaluated five days after challenge. In vitro results showed a dose-dependent cytotoxicity of Ag-NPs, which was highest for Ag50-polyvinilpyrrolidone (PVP), followed by Ag50-citrate, and lowest for Ag200-PVP. In vivo 10-50 µg Ag50-PVP triggered a dose-dependent pulmonary inflammatory milieu in NS and S mice, which was significantly higher in S mice and was dampened upon instillation of Ag200-PVP. Surprisingly, instillation of 1 µg Ag50-PVP significantly reduced OVA-induced inflammatory infiltrate in S mice and had no adverse effect in NS mice. Ag50-citrate showed similar beneficial effects at low concentrations and attenuated pro-inflammatory effects at high concentrations. The lung microbiome was altered by NPs instillation dependent on coating and/or mouse batch, showing the most pronounced effects upon instillation of 50 µg Ag50-citrate, which caused an increased abundance of operational taxonomic units assigned to Actinobacteria, Bacteroidetes, Firmicutes and Proteobacteria. However, no correlation with the biphasic effect of low and high Ag-NPs dose was found. Altogether, both in vitro and in vivo data on the pulmonary effects of Ag-NPs suggest the critical role of the size, dose and surface functionalization of Ag-NPs, especially in susceptible allergic individuals. From the perspective of occupational health, care should be taken by the production of Ag-NPs-containing consumer products.

Project description:The present study was carried out to reduce the size of silver nanoparticles (AgNPs) by optimizing physico-chemical conditions of the Aspergillus fumigatus BTCB10 growth based on central composite design (CCD) through response surface methodology (RSM). Variables such as a concentration of silver nitrate (mM), NaCl (%) and the wet weight of biomass (g) were controlled to produce spherical, monodispersed particles of 33.23 nm size, observing 78.7% reduction in size as compared to the initially obtained size that was equal to 356 nm. The obtained AgNPs exhibited negative zeta potential of -9.91 mV with a peak at 420 nm in the UV-Vis range whereas Fourier Transform Infrared (FT-IR) analysis identified O-H, C = C, C ≡ C, C-Br and C-Cl groups attached as capping agents. After conducting RSM experiments, a high nitrate reductase activity value of 179.15 nmol/h/ml was obtained; thus indicating a likely correlation between enzyme production and AgNPs synthesis. The F-value (significant at 3.91), non-significant lack of fit and determination coefficient (R2 = 0.7786) is representative of the good relation between the predicted values of response. We conclude that CCD is an effective tool in obtaining significant results of high quality and efficiency.The present study was carried out to reduce the size of silver nanoparticles (AgNPs) by optimizing physico-chemical conditions of the Aspergillus fumigatus BTCB10 growth based on central composite design (CCD) through response surface methodology (RSM). Variables such as a concentration of silver nitrate (mM), NaCl (%) and the wet weight of biomass (g) were controlled to produce spherical, monodispersed particles of 33.23 nm size, observing 78.7% reduction in size as compared to the initially obtained size that was equal to 356 nm. The obtained AgNPs exhibited negative zeta potential of –9.91 mV with a peak at 420 nm in the UV-Vis range whereas Fourier Transform Infrared (FT-IR) analysis identified O–H, C = C, C ≡ C, C–Br and C–Cl groups attached as capping agents. After conducting RSM experiments, a high nitrate reductase activity value of 179.15 nmol/h/ml was obtained; thus indicating a likely correlation between enzyme production and AgNPs synthesis. The F-value (significant at 3.91), non-significant lack of fit and determination coefficient (R2 = 0.7786) is representative of the good relation between the predicted values of response. We conclude that CCD is an effective tool in obtaining significant results of high quality and efficiency.

Dataset Information

Modulation of Human Macrophage Responses to Mycobacterium tuberculosis by Silver Nanoparticles of Different Size and Surface Modification.

Publications

Modulation of Human Macrophage Responses to Mycobacterium tuberculosis by Silver Nanoparticles of Different Size and Surface Modification.

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