Ontology highlight
ABSTRACT:
SUBMITTER: Sun N
PROVIDER: S-EPMC4656081 | biostudies-literature | 2015 Nov
REPOSITORIES: biostudies-literature
Sun Nuo N Yun Jeanho J Liu Jie J Malide Daniela D Liu Chengyu C Rovira Ilsa I II Holmström Kira M KM Fergusson Maria M MM Yoo Young Hyun YH Combs Christian A CA Finkel Toren T
Molecular cell 20151105 4
Alterations in mitophagy have been increasingly linked to aging and age-related diseases. There are, however, no convenient methods to analyze mitophagy in vivo. Here, we describe a transgenic mouse model in which we expressed a mitochondrial-targeted form of the fluorescent reporter Keima (mt-Keima). Keima is a coral-derived protein that exhibits both pH-dependent excitation and resistance to lysosomal proteases. Comparison of a wide range of primary cells and tissues generated from the mt-Keim ...[more]