Ontology highlight
ABSTRACT:
SUBMITTER: Li D
PROVIDER: S-EPMC4659358 | biostudies-literature | 2015 Aug
REPOSITORIES: biostudies-literature
Li Dong D Shao Lin L Chen Bi-Chang BC Zhang Xi X Zhang Mingshu M Moses Brian B Milkie Daniel E DE Beach Jordan R JR Hammer John A JA Pasham Mithun M Kirchhausen Tomas T Baird Michelle A MA Davidson Michael W MW Xu Pingyong P Betzig Eric E
Science (New York, N.Y.) 20150801 6251
Super-resolution fluorescence microscopy is distinct among nanoscale imaging tools in its ability to image protein dynamics in living cells. Structured illumination microscopy (SIM) stands out in this regard because of its high speed and low illumination intensities, but typically offers only a twofold resolution gain. We extended the resolution of live-cell SIM through two approaches: ultrahigh numerical aperture SIM at 84-nanometer lateral resolution for more than 100 multicolor frames, and no ...[more]