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Spherical nanoparticle supported lipid bilayers for the structural study of membrane geometry-sensitive molecules.

ABSTRACT: Many essential cellular processes including endocytosis and vesicle trafficking require alteration of membrane geometry. These changes are usually mediated by proteins that can sense and/or induce membrane curvature. Using spherical nanoparticle supported lipid bilayers (SSLBs), we characterize how SpoVM, a bacterial development factor, interacts with differently curved membranes by magic angle spinning solid-state NMR. Our results demonstrate that SSLBs are an effective system for structural and topological studies of membrane geometry-sensitive molecules.

SUBMITTER: Fu R

PROVIDER: S-EPMC4677685 | biostudies-literature | 2015 Nov

REPOSITORIES: biostudies-literature

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Spherical nanoparticle supported lipid bilayers for the structural study of membrane geometry-sensitive molecules.

Fu Riqiang R Gill Richard L RL Kim Edward Y EY Briley Nicole E NE Tyndall Erin R ER Xu Jie J Li Conggang C Ramamurthi Kumaran S KS Flanagan John M JM Tian Fang F

Journal of the American Chemical Society 20151028 44

Many essential cellular processes including endocytosis and vesicle trafficking require alteration of membrane geometry. These changes are usually mediated by proteins that can sense and/or induce membrane curvature. Using spherical nanoparticle supported lipid bilayers (SSLBs), we characterize how SpoVM, a bacterial development factor, interacts with differently curved membranes by magic angle spinning solid-state NMR. Our results demonstrate that SSLBs are an effective system for structural an ...[more]

PMID: 26488086

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