Unknown

Dataset Information

0

The Histone Demethylase KDM5 Activates Gene Expression by Recognizing Chromatin Context through Its PHD Reader Motif.


ABSTRACT: KDM5 family proteins are critically important transcriptional regulators whose physiological functions in the context of a whole animal remain largely unknown. Using genome-wide gene expression and binding analyses in Drosophila adults, we demonstrate that KDM5 (Lid) is a direct regulator of genes required for mitochondrial structure and function. Significantly, this occurs independently of KDM5's well-described JmjC domain-encoded histone demethylase activity. Instead, it requires the PHD motif of KDM5 that binds to histone H3 that is di- or trimethylated on lysine 4 (H3K4me2/3). Genome-wide, KDM5 binding overlaps with the active chromatin mark H3K4me3, and a fly strain specifically lacking H3K4me2/3 binding shows defective KDM5 promoter recruitment and gene activation. KDM5 therefore plays a central role in regulating mitochondrial function by utilizing its ability to recognize specific chromatin contexts. Importantly, KDM5-mediated regulation of mitochondrial activity is likely to be key in human diseases caused by dysfunction of this family of proteins.

SUBMITTER: Liu X 

PROVIDER: S-EPMC4684901 | biostudies-literature | 2015 Dec

REPOSITORIES: biostudies-literature

altmetric image

Publications

The Histone Demethylase KDM5 Activates Gene Expression by Recognizing Chromatin Context through Its PHD Reader Motif.

Liu Xingyin X   Secombe Julie J  

Cell reports 20151207 10


KDM5 family proteins are critically important transcriptional regulators whose physiological functions in the context of a whole animal remain largely unknown. Using genome-wide gene expression and binding analyses in Drosophila adults, we demonstrate that KDM5 (Lid) is a direct regulator of genes required for mitochondrial structure and function. Significantly, this occurs independently of KDM5's well-described JmjC domain-encoded histone demethylase activity. Instead, it requires the PHD motif  ...[more]

Similar Datasets

| S-EPMC7058283 | biostudies-literature
| S-EPMC6310147 | biostudies-literature
| S-EPMC6028249 | biostudies-literature
| S-EPMC5177622 | biostudies-literature
| S-EPMC5854480 | biostudies-literature
| S-EPMC6749836 | biostudies-literature
| S-EPMC4905292 | biostudies-other
| S-EPMC6979384 | biostudies-literature
| S-EPMC4018760 | biostudies-literature
| S-EPMC4975413 | biostudies-literature