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Continuous enrichment of low-abundance cell samples using standing surface acoustic waves (SSAW).

ABSTRACT: Cell enrichment is a powerful tool in a variety of cellular studies, especially in applications with low-abundance cell types. In this work, we developed a standing surface acoustic wave (SSAW) based microfluidic device for non-contact, continuous cell enrichment. With a pair of parallel interdigital transducers (IDT) deposited on a piezoelectric substrate, a one-dimensional SSAW field was established along disposable micro-tubing channels, generating numerous pressure nodes (and thus numerous cell-enrichment regions). Our method is able to concentrate highly diluted blood cells by more than 100 fold with a recovery efficiency of up to 99%. Such highly effective cell enrichment was achieved without using sheath flow. The SSAW-based technique presented here is simple, bio-compatible, label-free, and sheath-flow-free. With these advantages, it could be valuable for many biomedical applications.

SUBMITTER: Chen Y

PROVIDER: S-EPMC4688895 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature

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Continuous enrichment of low-abundance cell samples using standing surface acoustic waves (SSAW).

Chen Yuchao Y Li Sixing S Gu Yeyi Y Li Peng P Ding Xiaoyun X Wang Lin L McCoy J Philip JP Levine Stewart J SJ Huang Tony Jun TJ

Lab on a chip 20140301 5

Cell enrichment is a powerful tool in a variety of cellular studies, especially in applications with low-abundance cell types. In this work, we developed a standing surface acoustic wave (SSAW) based microfluidic device for non-contact, continuous cell enrichment. With a pair of parallel interdigital transducers (IDT) deposited on a piezoelectric substrate, a one-dimensional SSAW field was established along disposable micro-tubing channels, generating numerous pressure nodes (and thus numerous c ...[more]

PMID: 24413889

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