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Standing surface acoustic wave (SSAW)-based cell washing.

ABSTRACT: Cell/bead washing is an indispensable sample preparation procedure used in various cell studies and analytical processes. In this article, we report a standing surface acoustic wave (SSAW)-based microfluidic device for cell and bead washing in a continuous flow. In our approach, the acoustic radiation force generated in a SSAW field is utilized to actively extract cells or beads from their original medium. A unique configuration of tilted-angle standing surface acoustic wave (taSSAW) is employed in our device, enabling us to wash beads with >98% recovery rate and >97% washing efficiency. We also demonstrate the functionality of our device by preparing high-purity (>97%) white blood cells from lysed blood samples through cell washing. Our SSAW-based cell/bead washing device has the advantages of label-free manipulation, simplicity, high biocompatibility, high recovery rate, and high washing efficiency. It can be useful for many lab-on-a-chip applications.

SUBMITTER: Li S

PROVIDER: S-EPMC4442640 | biostudies-literature | 2015 Jan

REPOSITORIES: biostudies-literature

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Standing surface acoustic wave (SSAW)-based cell washing.

Li Sixing S Ding Xiaoyun X Mao Zhangming Z Chen Yuchao Y Nama Nitesh N Guo Feng F Li Peng P Wang Lin L Cameron Craig E CE Huang Tony Jun TJ

Lab on a chip 20150101 1

Cell/bead washing is an indispensable sample preparation procedure used in various cell studies and analytical processes. In this article, we report a standing surface acoustic wave (SSAW)-based microfluidic device for cell and bead washing in a continuous flow. In our approach, the acoustic radiation force generated in a SSAW field is utilized to actively extract cells or beads from their original medium. A unique configuration of tilted-angle standing surface acoustic wave (taSSAW) is employed ...[more]

PMID: 25372273

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