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?XANES: In vivo imaging of metal-protein coordination environments.


ABSTRACT: We have developed an X-ray absorption near edge structure spectroscopy method using fluorescence detection for visualizing in vivo coordination environments of metals in biological specimens. This approach, which we term fluorescence imaging XANES (?XANES), allows us to spatially depict metal-protein associations in a native, hydrated state whilst avoiding intrinsic chemical damage from radiation. This method was validated using iron-challenged Caenorhabditis elegans to observe marked alterations in redox environment.

SUBMITTER: James SA 

PROVIDER: S-EPMC4748213 | biostudies-literature | 2016 Feb

REPOSITORIES: biostudies-literature

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φXANES: In vivo imaging of metal-protein coordination environments.

James Simon A SA   Hare Dominic J DJ   Jenkins Nicole L NL   de Jonge Martin D MD   Bush Ashley I AI   McColl Gawain G  

Scientific reports 20160210


We have developed an X-ray absorption near edge structure spectroscopy method using fluorescence detection for visualizing in vivo coordination environments of metals in biological specimens. This approach, which we term fluorescence imaging XANES (φXANES), allows us to spatially depict metal-protein associations in a native, hydrated state whilst avoiding intrinsic chemical damage from radiation. This method was validated using iron-challenged Caenorhabditis elegans to observe marked alteration  ...[more]

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