Ontology highlight
ABSTRACT:
SUBMITTER: James SA
PROVIDER: S-EPMC4748213 | biostudies-literature | 2016 Feb
REPOSITORIES: biostudies-literature
James Simon A SA Hare Dominic J DJ Jenkins Nicole L NL de Jonge Martin D MD Bush Ashley I AI McColl Gawain G
Scientific reports 20160210
We have developed an X-ray absorption near edge structure spectroscopy method using fluorescence detection for visualizing in vivo coordination environments of metals in biological specimens. This approach, which we term fluorescence imaging XANES (φXANES), allows us to spatially depict metal-protein associations in a native, hydrated state whilst avoiding intrinsic chemical damage from radiation. This method was validated using iron-challenged Caenorhabditis elegans to observe marked alteration ...[more]