Ontology highlight
ABSTRACT:
SUBMITTER: Bottanelli F
PROVIDER: S-EPMC4785223 | biostudies-literature | 2016 Mar
REPOSITORIES: biostudies-literature
Bottanelli Francesca F Kromann Emil B EB Allgeyer Edward S ES Erdmann Roman S RS Wood Baguley Stephanie S Sirinakis George G Schepartz Alanna A Baddeley David D Toomre Derek K DK Rothman James E JE Bewersdorf Joerg J
Nature communications 20160304
Stimulated emission depletion (STED) nanoscopy allows observations of subcellular dynamics at the nanoscale. Applications have, however, been severely limited by the lack of a versatile STED-compatible two-colour labelling strategy for intracellular targets in living cells. Here we demonstrate a universal labelling method based on the organic, membrane-permeable dyes SiR and ATTO590 as Halo and SNAP substrates. SiR and ATTO590 constitute the first suitable dye pair for two-colour STED imaging in ...[more]