Unknown

Dataset Information

0

Two-colour live-cell nanoscale imaging of intracellular targets.


ABSTRACT: Stimulated emission depletion (STED) nanoscopy allows observations of subcellular dynamics at the nanoscale. Applications have, however, been severely limited by the lack of a versatile STED-compatible two-colour labelling strategy for intracellular targets in living cells. Here we demonstrate a universal labelling method based on the organic, membrane-permeable dyes SiR and ATTO590 as Halo and SNAP substrates. SiR and ATTO590 constitute the first suitable dye pair for two-colour STED imaging in living cells below 50?nm resolution. We show applications with mitochondria, endoplasmic reticulum, plasma membrane and Golgi-localized proteins, and demonstrate continuous acquisition for up to 3?min at 2-s time resolution.

SUBMITTER: Bottanelli F 

PROVIDER: S-EPMC4785223 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

altmetric image

Publications


Stimulated emission depletion (STED) nanoscopy allows observations of subcellular dynamics at the nanoscale. Applications have, however, been severely limited by the lack of a versatile STED-compatible two-colour labelling strategy for intracellular targets in living cells. Here we demonstrate a universal labelling method based on the organic, membrane-permeable dyes SiR and ATTO590 as Halo and SNAP substrates. SiR and ATTO590 constitute the first suitable dye pair for two-colour STED imaging in  ...[more]

Similar Datasets

| S-EPMC2702483 | biostudies-literature
| S-EPMC10374873 | biostudies-literature
| S-EPMC4482072 | biostudies-literature
| S-EPMC4915154 | biostudies-literature
| S-EPMC4156865 | biostudies-literature
| S-EPMC3800097 | biostudies-literature
| S-EPMC7061308 | biostudies-literature
| S-EPMC5815523 | biostudies-literature
| S-EPMC2954500 | biostudies-literature
| S-EPMC4467025 | biostudies-literature