Ontology highlight
ABSTRACT:
SUBMITTER: Leenay RT
PROVIDER: S-EPMC4826307 | biostudies-literature | 2016 Apr
REPOSITORIES: biostudies-literature
Leenay Ryan T RT Maksimchuk Kenneth R KR Slotkowski Rebecca A RA Agrawal Roma N RN Gomaa Ahmed A AA Briner Alexandra E AE Barrangou Rodolphe R Beisel Chase L CL
Molecular cell 20160331 1
CRISPR-Cas adaptive immune systems in prokaryotes boast a diversity of protein families and mechanisms of action, where most systems rely on protospacer-adjacent motifs (PAMs) for DNA target recognition. Here, we developed an in vivo, positive, and tunable screen termed PAM-SCANR (PAM screen achieved by NOT-gate repression) to elucidate functional PAMs as well as an interactive visualization scheme termed the PAM wheel to convey individual PAM sequences and their activities. PAM-SCANR and the PA ...[more]