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Protein crystal screening and characterization for serial femtosecond nanocrystallography.

ABSTRACT: The recent development of X-ray free electron lasers (XFELs) has spurred the development of serial femtosecond nanocrystallography (SFX) which, for the first time, is enabling structure retrieval from sub-micron protein crystals. Although there are already a growing number of structures published using SFX, the technology is still very new and presents a number of unique challenges as well as opportunities for structural biologists. One of the biggest barriers to the success of SFX experiments is the preparation and selection of suitable protein crystal samples. Here we outline a protocol for preparing and screening for suitable XFEL targets.

SUBMITTER: Darmanin C

PROVIDER: S-EPMC4853777 | biostudies-literature | 2016 May

REPOSITORIES: biostudies-literature

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Protein crystal screening and characterization for serial femtosecond nanocrystallography.

Darmanin Connie C Strachan Jamie J Adda Christopher G CG Ve Thomas T Kobe Bostjan B Abbey Brian B

Scientific reports 20160503

The recent development of X-ray free electron lasers (XFELs) has spurred the development of serial femtosecond nanocrystallography (SFX) which, for the first time, is enabling structure retrieval from sub-micron protein crystals. Although there are already a growing number of structures published using SFX, the technology is still very new and presents a number of unique challenges as well as opportunities for structural biologists. One of the biggest barriers to the success of SFX experiments i ...[more]

PMID: 27139248

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