Ontology highlight
ABSTRACT:
SUBMITTER: Yamano T
PROVIDER: S-EPMC4899970 | biostudies-literature | 2016 May
REPOSITORIES: biostudies-literature
Cell 20160421 4
Cpf1 is an RNA-guided endonuclease of a type V CRISPR-Cas system that has been recently harnessed for genome editing. Here, we report the crystal structure of Acidaminococcus sp. Cpf1 (AsCpf1) in complex with the guide RNA and its target DNA at 2.8 Å resolution. AsCpf1 adopts a bilobed architecture, with the RNA-DNA heteroduplex bound inside the central channel. The structural comparison of AsCpf1 with Cas9, a type II CRISPR-Cas nuclease, reveals both striking similarity and major differences, t ...[more]