Ontology highlight
ABSTRACT:
SUBMITTER: Inoue R
PROVIDER: S-EPMC4933968 | biostudies-literature | 2016 Jul
REPOSITORIES: biostudies-literature
Inoue Rintaro R Takata Takumi T Fujii Norihiko N Ishii Kentaro K Uchiyama Susumu S Sato Nobuhiro N Oba Yojiro Y Wood Kathleen K Kato Koichi K Fujii Noriko N Sugiyama Masaaki M
Scientific reports 20160706
α-Crystallin possesses a dynamic quaternary structure mediated by its subunit dynamics. Elucidation of a mechanism of subunit dynamics in homo-oligomers of αB-crystallin was tackled through deuteration-assisted small-angle neutron scattering (DA-SANS) and electrospray ionization (ESI) native mass spectrometry (nMS). The existence of subunit exchange was confirmed with DA-SANS, and monomers liberated from the oligomers were observed with nMS. With increasing temperature, an increase in both the e ...[more]