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Glycation Reactivity of a Quorum-Sensing Signaling Molecule.

ABSTRACT: Reported herein is that (4S)-4,5-dihydroxy-2,3-pentanedione (DPD) can undergo a previously undocumented non-enzymatic glycation reaction. Incubation of DPD with viral DNA or the antibiotic gramicidin?S resulted in significant biochemical alterations. A protein-labeling method was consequently developed that facilitated the identification of unrecognized glycation targets of DPD in a prokaryotic system. These results open new avenues toward tracking and understanding the fate and function of the elusive quorum-sensing signaling molecule.

SUBMITTER: Tsuchikama K

PROVIDER: S-EPMC4965832 | biostudies-literature | 2016 Mar

REPOSITORIES: biostudies-literature

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Glycation Reactivity of a Quorum-Sensing Signaling Molecule.

Tsuchikama Kyoji K Gooyit Major M Harris Tyler L TL Zhu Jie J Globisch Daniel D Kaufmann Gunnar F GF Janda Kim D KD

Angewandte Chemie (International ed. in English) 20160217 12

Reported herein is that (4S)-4,5-dihydroxy-2,3-pentanedione (DPD) can undergo a previously undocumented non-enzymatic glycation reaction. Incubation of DPD with viral DNA or the antibiotic gramicidin S resulted in significant biochemical alterations. A protein-labeling method was consequently developed that facilitated the identification of unrecognized glycation targets of DPD in a prokaryotic system. These results open new avenues toward tracking and understanding the fate and function of the ...[more]

PMID: 26890076

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