Ontology highlight
ABSTRACT:
SUBMITTER: Hollywood JA
PROVIDER: S-EPMC4997560 | biostudies-literature | 2016 Aug
REPOSITORIES: biostudies-literature
Hollywood Jennifer A JA Lee Ciaran M CM Scallan Martina F MF Harrison Patrick T PT
Scientific reports 20160825
To maximise the efficiency of template-dependent gene editing, most studies describe programmable and/or RNA-guided endonucleases that make a double-stranded break at, or close to, the target sequence to be modified. The rationale for this design strategy is that most gene repair tracts will be very short. Here, we describe a CRISPR Cas9/gRNA selection-free strategy which uses deep sequencing to characterise repair tracts from a donor plasmid containing seven nucleotide differences across a 216 ...[more]