Ontology highlight
ABSTRACT:
SUBMITTER: Pinto C
PROVIDER: S-EPMC5030094 | biostudies-literature | 2016 Sep
REPOSITORIES: biostudies-literature
Pinto Cosimo C Kasaciunaite Kristina K Seidel Ralf R Cejka Petr P
eLife 20160909
Human DNA2 (hDNA2) contains both a helicase and a nuclease domain within the same polypeptide. The nuclease of hDNA2 is involved in a variety of DNA metabolic processes. Little is known about the role of the hDNA2 helicase. Using bulk and single-molecule approaches, we show that hDNA2 is a processive helicase capable of unwinding kilobases of dsDNA in length. The nuclease activity prevents the engagement of the helicase by competing for the same substrate, hence prominent DNA unwinding by hDNA2 ...[more]