Oriented collagen fibers direct tumor cell intravasation.
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ABSTRACT:
SUBMITTER: Han W
PROVIDER: S-EPMC5056065 | biostudies-literature | 2016 Oct
REPOSITORIES: biostudies-literature
Publications
Oriented collagen fibers direct tumor cell intravasation.
Han Weijing W Chen Shaohua S Yuan Wei W Fan Qihui Q Tian Jianxiang J Wang Xiaochen X Chen Longqing L Zhang Xixiang X Wei Weili W Liu Ruchuan R Qu Junle J Jiao Yang Y Austin Robert H RH Liu Liyu L
Proceedings of the National Academy of Sciences of the United States of America 20160923 40
In this work, we constructed a Collagen I-Matrigel composite extracellular matrix (ECM). The composite ECM was used to determine the influence of the local collagen fiber orientation on the collective intravasation ability of tumor cells. We found that the local fiber alignment enhanced cell-ECM interactions. Specifically, metastatic MDA-MB-231 breast cancer cells followed the local fiber alignment direction during the intravasation into rigid Matrigel (∼10 mg/mL protein concentration). ...[more]
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