Ontology highlight
ABSTRACT:
SUBMITTER: Jia D
PROVIDER: S-EPMC5105194 | biostudies-literature | 2016 Nov
REPOSITORIES: biostudies-literature
Nature communications 20161109
Retromer is a membrane coat complex that is recruited to endosomes by the small GTPase Rab7 and sorting nexin 3. The timing of this interaction and consequent endosomal dynamics are thought to be regulated by the guanine nucleotide cycle of Rab7. Here we demonstrate that TBC1d5, a GTPase-activating protein (GAP) for Rab7, is a high-affinity ligand of the retromer cargo selective complex VPS26/VPS29/VPS35. The crystal structure of the TBC1d5 GAP domain bound to VPS29 and complementary biochemical ...[more]