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Single-Molecule Fluorescence Detection of a Synthetic Heparan Sulfate Disaccharide.

ABSTRACT: The first single-molecule fluorescence detection of a structurally-defined synthetic carbohydrate is reported: a heparan sulfate (HS) disaccharide fragment labeled with Alexa488. Single molecules have been measured whilst freely diffusing in solution and controlled encapsulation in surface-tethered lipid vesicles has allowed extended observations of carbohydrate molecules down to the single-molecule level. The diverse and dynamic nature of HS-protein interactions means that new tools to investigate pure HS fragments at the molecular level would significantly enhance our understanding of HS. This work is a proof-of-principle demonstration of the feasibility of single-molecule studies of synthetic carbohydrates which offers a new approach to the study of pure glycosaminoglycan (GAG) fragments.

SUBMITTER: Dalton CE

PROVIDER: S-EPMC5111599 | biostudies-literature | 2016 Nov

REPOSITORIES: biostudies-literature

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Single-Molecule Fluorescence Detection of a Synthetic Heparan Sulfate Disaccharide.

Dalton Charlotte E CE Quinn Steven D SD Rafferty Aidan A Morten Michael J MJ Gardiner John M JM Magennis Steven W SW

Chemphyschem : a European journal of chemical physics and physical chemistry 20160914 21

The first single-molecule fluorescence detection of a structurally-defined synthetic carbohydrate is reported: a heparan sulfate (HS) disaccharide fragment labeled with Alexa488. Single molecules have been measured whilst freely diffusing in solution and controlled encapsulation in surface-tethered lipid vesicles has allowed extended observations of carbohydrate molecules down to the single-molecule level. The diverse and dynamic nature of HS-protein interactions means that new tools to investig ...[more]

PMID: 27538128

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