Ontology highlight
ABSTRACT:
SUBMITTER: Brieba LG
PROVIDER: S-EPMC516626 | biostudies-literature | 2004 Sep
REPOSITORIES: biostudies-literature
Brieba Luis G LG Eichman Brandt F BF Kokoska Robert J RJ Doublié Sylvie S Kunkel Tom A TA Ellenberger Tom T
The EMBO journal 20040805 17
Accurate DNA replication involves polymerases with high nucleotide selectivity and proofreading activity. We show here why both fidelity mechanisms fail when normally accurate T7 DNA polymerase bypasses the common oxidative lesion 8-oxo-7, 8-dihydro-2'-deoxyguanosine (8oG). The crystal structure of the polymerase with 8oG templating dC insertion shows that the O8 oxygen is tolerated by strong kinking of the DNA template. A model of a corresponding structure with dATP predicts steric and electros ...[more]