Dataset Information

The role of polymorphism of interleukin-2, -10, -13 and TNF-? genes in cutaneous T-cell lymphoma pathogenesis.

ABSTRACT: INTRODUCTION:As the pathogenesis of cutaneous T-cell lymphomas (CTCL) is not fully understood, inherited gene polymorphisms are considered to play a role in the development of lymphomas. AIM:To investigate whether certain gene polymorphisms might be involved in the development of CTCL. MATERIAL AND METHODS:In the case-control study we compared the frequency of nine selected single nucleotide polymorphisms (SNP) of seven genes (rs1800587/-889 C/T of interleukin (IL)-1?, rs2069762/-330G/T) and rs2069763/+166G/T of IL-2, rs1800925/-1112 C/T of IL-13, rs1800896/-1082 A/G of IL-10, rs4073/-251 A/T of IL-8, rs5370/K198N, rs180054/-1370T/G of endothelin-1 and rs1800629/-308 G/A of TNF-?) in 43 CTCL and Polish cases using the amplification refractory mutation system polymerase chain reaction method. RESULTS:We have found that two genotypes, -330GG of IL-2 and -1112TT of IL-13 both promoter variants associated with "hypertranscription phenotype", were over-represented in CTCL patients compared to healthy controls, and they increase the risk of malignancy development (OR = 5.82, p = 0.001 for IL-2 -330 GG, and OR = 5.67, p = 0.0024 for IL-13 -1112 TT). On the other hand, high transcription -308A allele of the TNF-? gene and -1082GG of IL-10 genotype is less frequent in lymphoma patients and has protective effects on the development of CTCL (OR = 0.45, p = 0.0466 for -308A of TNF-?, and OR = 0.35, p = 0.0329 for -1082GG of IL-10 genes). CONCLUSIONS:Our results indicate that hypertranscription promoter variants of IL-2 and IL-13 genes could be estimated as the risk factor for development of CTCL, while TNF-? and IL-10 variants have a protective effect.

SUBMITTER: Nedoszytko B

PROVIDER: S-EPMC5183781 | biostudies-literature | 2016 Dec

REPOSITORIES: biostudies-literature

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The role of polymorphism of <i>interleukin-2</i>, -<i>10</i>, <i>-13</i> and <i>TNF-</i>α genes in cutaneous T-cell lymphoma pathogenesis.

Nedoszytko Boguslaw B Olszewska Berenika B Roszkiewicz Jadwiga J Glen Jolanta J Zabłotna Monika M Ługowska-Umer Hanna H Nowicki Roman R Sokołowska-Wojdyło Małgorzata M

Postepy dermatologii i alergologii 20161202 6

<h4>Introduction</h4>As the pathogenesis of cutaneous T-cell lymphomas (CTCL) is not fully understood, inherited gene polymorphisms are considered to play a role in the development of lymphomas.<h4>Aim</h4>To investigate whether certain gene polymorphisms might be involved in the development of CTCL.<h4>Material and methods</h4>In the case-control study we compared the frequency of nine selected single nucleotide polymorphisms (SNP) of seven genes (<i>rs1800587/-889 C/T of interleukin (IL)-1</i> ...[more]

PMID: 28035219

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Project description:Eimeria species are intestinal protozoan parasites that cause lack of production, malabsorption and mortality in floor raised chickens. Administering an oral antibody to interleukin 10 (aIL-10) reduces the symptoms of coccidiosis in broilers, indicating interleukin 10 (IL-10) is key to Eimeria pathology. IL-10 is an anti-inflammatory cytokine and acts as a stand down signal to reduce inflammation and host pathology during disease. Related protozoan parasites exploit IL-10 to reduce pathogen-damaging host inflammatory responses. We hypothesize that IL-10 is increased during Eimeria infection through an unknown host-pathogen interaction, and by feeding aIL-10 to neutralize excess IL-10 the bird is allowed to mount an effective immune response to Eimeria. To determine the effects of aIL-10 during the intestinal immune response, intestinal pathology and the relationship between IL-10, interferon gamma (IFNγ) and Eimeria infection were evaluated in this study. In both experiments, broilers were administered either a 10x dose of Advent® Eimeria vaccine or saline. Duodenum, jejunum and cecum samples were collected, processed, stained and examined under a microscope. Evaluation of intestinal histomorphology during aIL-10 administration showed minimal differences in birds fed aIL-10 during infection compared to animals fed a control antibody during Eimeria infection. To further evaluate aIL-10's positive effect during infection, immunofluorescent histochemistry was performed on chicken intestines days 3-7 post Eimeria infection for IL-10 and IFNγ presence in intestinal mucosa in control and infected birds, in regions with and without visible Eimeria burden. IL-10 and IFNγ had significant changes between days 4.5-7 post-infection in birds fed aIL-10 compared to animals fed a control antibody. Overall we found that the duodenum had increased IL-10 presence and increased IFNγ presence, and the jejunum and cecum had decreased IL-10 presence and decreased IFNγ presence. These differences in spatial regulation of IL-10 and IFNγ may indicate Eimeria species induce slightly different cytokine responses.

Dataset Information

The role of polymorphism of interleukin-2, -10, -13 and TNF-? genes in cutaneous T-cell lymphoma pathogenesis.

Publications

The role of polymorphism of <i>interleukin-2</i>, -<i>10</i>, <i>-13</i> and <i>TNF-</i>α genes in cutaneous T-cell lymphoma pathogenesis.

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