Protein evolution by codon-based random deletions.
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ABSTRACT:
SUBMITTER: Osuna J
PROVIDER: S-EPMC521680 | biostudies-literature | 2004 Sep
REPOSITORIES: biostudies-literature
Publications
Protein evolution by codon-based random deletions.
Osuna Joel J Yáñez Jorge J Soberón Xavier X Gaytán Paul P
Nucleic acids research 20040930 17
A method to delete in-phase codons throughout a defined target region of a gene has been developed. This approach, named the codon-based random deletion (COBARDE) method, is able to delete complete codons in a random and combinatorial mode. Robustness, automation and fine-tuning of the mutagenesis rate are essential characteristics of the method, which is based on the assembly of oligonucleotides and on the use of two transient orthogonal protecting groups during the chemical synthesis. The perf ...[more]
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