Dataset Information

Mild hyperglycemia triggered islet function recovery in streptozotocin-induced insulin-deficient diabetic rats.

ABSTRACT:

Aims/introduction

Moderate elevation of glucose level has been shown to effectively promote β-cell replication in various models in vitro and in normal rodents. Here, we aimed to test the effect of moderately elevated glucose on β-cell mass expansion and islet function recovery in diabetic animal models.

Materials and methods

A single high dose of streptozotocin was given to induce insulin-deficient diabetes in adult male Sprague-Dawley rats. Then, 48 h after streptozotocin injection, newly diabetic rats were randomly divided into three groups: (i) no treatment to maintain hyperglycemia; (ii) daily exogenous long-acting human insulin analog injection that maintained mild hyperglycemia (15 mmol/L < blood glucose < 18 mmol/L); (iii) daily exogenous long-acting human insulin analog injection to restore normoglycemia (blood glucose <8 mmol/L) as a control. Islet function, β-cell regeneration and β-cell replication were monitored during the entire analysis period.

Results

A single high dose of streptozotocin induced massive loss of β-cells, resulting in irreversible hyperglycemia. Mild hyperglycemia markedly promoted β-cell proliferation, leading to robust β-cell regeneration. Importantly, rats that maintained mild hyperglycemia showed nearly normal glucose-stimulated insulin secretion, glucose disposal and random blood glucose levels, suggesting almost full restoration of the islet function. Normalization of blood glucose levels profoundly blunted β-cell replication, regeneration and islet function recovery observed in mild hyperglycemia.

Conclusions

Our research provides a feasible approach to stimulate in situ β-cell regeneration in diabetic rats, offering new perspectives for diabetes therapy.

SUBMITTER: Cheng Y

PROVIDER: S-EPMC5217940 | biostudies-literature | 2017 Jan

REPOSITORIES: biostudies-literature

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Publications

Mild hyperglycemia triggered islet function recovery in streptozotocin-induced insulin-deficient diabetic rats.

Cheng Yu Y Shen Jing J Ren Weizheng W Hao Haojie H Xie Zongyan Z Liu Jiejie J Mu Yiming Y Han Weidong W

Journal of diabetes investigation 20160708 1

<h4>Aims/introduction</h4>Moderate elevation of glucose level has been shown to effectively promote β-cell replication in various models in vitro and in normal rodents. Here, we aimed to test the effect of moderately elevated glucose on β-cell mass expansion and islet function recovery in diabetic animal models.<h4>Materials and methods</h4>A single high dose of streptozotocin was given to induce insulin-deficient diabetes in adult male Sprague-Dawley rats. Then, 48 h after streptozotocin inject ...[more]

PMID: 27184687

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Dataset Information

Mild hyperglycemia triggered islet function recovery in streptozotocin-induced insulin-deficient diabetic rats.

Aims/introduction

Materials and methods

Results

Conclusions

Publications

Mild hyperglycemia triggered islet function recovery in streptozotocin-induced insulin-deficient diabetic rats.

Similar Datasets

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