Ontology highlight
ABSTRACT:
SUBMITTER: Horii T
PROVIDER: S-EPMC5380110 | biostudies-literature | 2014 Mar
REPOSITORIES: biostudies-literature
Horii Takuro T Arai Yuji Y Yamazaki Miho M Morita Sumiyo S Kimura Mika M Itoh Masahiro M Abe Yumiko Y Hatada Izuho I
Scientific reports 20140328
The CRISPR/Cas system, in which the Cas9 endonuclease and a guide RNA complementary to the target are sufficient for RNA-guided cleavage of the target DNA, is a powerful new approach recently developed for targeted gene disruption in various animal models. However, there is little verification of microinjection methods for generating knockout mice using this approach. Here, we report the verification of microinjection methods of the CRISPR/Cas system. We compared three methods for injection: (1) ...[more]