Dataset Information

Telomere shortening and accelerated aging in COPD: findings from the BODE cohort.

ABSTRACT:

Background

Chronic Obstructive Pulmonary Disease (COPD) may be associated with accelerated aging. Telomere shortening is a biomarker of aging. Cross-sectional studies describe shorter telomeres in COPD compared with matched controls. No studies have described telomere length trajectory and its relationship with COPD progression. We investigated telomere shortening over time and its relationship to clinical and lung function parameters in a COPD cohort and smoker controls without COPD.

Methods

At baseline leukocyte telomere length was measured by qPCR in 121 smokers with COPD and 121 without COPD matched by age (T/S₀). The measurements were repeated in 70 of those patients with COPD and 73 non-COPD smokers after 3 years of follow up (T/S₃).

Results

At initial measurement, telomeres were shorter in COPD patients when compared to smoker controls (T/S?=?0.68?±?0.25 vs. 0.88?±?0.52, p?=?0.003) independent from age and sex. During the follow-up period, we observed an accelerated telomere shortening in individuals with COPD in contrast to smoker controls (T/S₀?=?0.66?±?0.21 vs. T/S₃?=?0.46?±?0.16, p?0?=?0.83?±?0.56 vs. T/S₃?=?0.74?±?0.52, p?=?0.023 for controls; GLIM, p?=?0.001). This shortening was inversely related to the baseline telomere length (r?=?-0.49, p??0.05).

Conclusions

Compared with smokers, patients with COPD have accelerated telomere shortening and this rate of attrition depends on baseline telomere length. Furthermore, the telomere length and its rate of shortening did not relate to clinical and lung function parameters changes over 3 years of follow-up.

SUBMITTER: Cordoba-Lanus E

PROVIDER: S-EPMC5390353 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature

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Publications

Telomere shortening and accelerated aging in COPD: findings from the BODE cohort.

Córdoba-Lanús Elizabeth E Cazorla-Rivero Sara S Espinoza-Jiménez Adriana A de-Torres Juan P JP Pajares María J MJ Aguirre-Jaime Armando A Celli Bartolomé B Casanova Ciro C

Respiratory research 20170413 1

<h4>Background</h4>Chronic Obstructive Pulmonary Disease (COPD) may be associated with accelerated aging. Telomere shortening is a biomarker of aging. Cross-sectional studies describe shorter telomeres in COPD compared with matched controls. No studies have described telomere length trajectory and its relationship with COPD progression. We investigated telomere shortening over time and its relationship to clinical and lung function parameters in a COPD cohort and smoker controls without COPD.<h4 ...[more]

PMID: 28407775

Similar Datasets

Project description:BACKGROUND:Telomere shortening has been associated with several lung diseases. However, telomere length is generally measured in peripheral blood leucocytes rather than in lung tissue, where disease occurs. Consequently, telomere dynamics have not been established for the normal human lung nor for diseased lung tissue. We hypothesized an age- and disease-dependent shortening of lung tissue telomeres. METHODS:At time of (re-)transplantation or autopsy, 70 explant lungs were collected: from unused donors (normal, n = 13) and patients with cystic fibrosis (CF, n = 12), chronic obstructive pulmonary disease (COPD, n = 11), chronic hypersensitivity pneumonitis (cHP, n = 9), bronchiolitis obliterans syndrome (BOS) after prior transplantation (n = 11) and restrictive allograft syndrome (RAS) after prior transplantation (n = 14). Lungs were inflated, frozen and then scanned using CT. Four tissue cores from distinct lung regions were sampled for analysis. Disease severity was evaluated using CT and micro CT imaging. DNA was extracted from the samples and average relative telomere length (RTL) was determined using real-time qPCR. RESULTS:The normal lungs showed a decrease in RTL with age (p < 0.0001). Of the diseased lungs, only BOS and RAS showed significant RTL decrease with increasing lung age (p = 0.0220 and p = 0.0272 respectively). Furthermore, we found that RTL showed considerable variability between samples within both normal and diseased lungs. cHP, BOS and RAS lungs had significant shorter RTL in comparison with normal lungs, after adjustment for lung age, sex and BMI (p < 0.0001, p = 0.0051 and p = 0.0301 respectively). When investigating the relation between RTL and regional disease severity in CF, cHP and RAS, no association was found. CONCLUSION:These results show a progressive decline in telomere length with age in normal, BOS and RAS lungs. cHP, BOS and RAS lungs demonstrated shorter RTL compared to normal lungs. Lung tissue RTL does not associate with regional disease severity within the lung. Therefore, tissue RTL does not seem to fully reflect peripheral blood telomere length.

Dataset Information

Telomere shortening and accelerated aging in COPD: findings from the BODE cohort.

Background

Methods

Results

Conclusions

Publications

Telomere shortening and accelerated aging in COPD: findings from the BODE cohort.

Similar Datasets

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