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ADP-ribosyl cyclases regulate early development of the sea urchin.

ABSTRACT: ADP-ribosyl cyclases are multifunctional enzymes involved in the metabolism of nucleotide derivatives necessary for Ca2+ signalling such as cADPR and NAADP. Although Ca2+ signalling is a critical regulator of early development, little is known of the role of ADP-ribosyl cyclases during embryogenesis. Here we analyze the expression, activity and function of ADP-ribosyl cyclases in the embryo of the sea urchin - a key organism for study of both Ca2+ signalling and embryonic development. ADP-ribosyl cyclase isoforms (SpARC1-4) showed unique changes in expression during early development. These changes were associated with an increase in the ratio of cADPR:NAADP production. Over-expression of SpARC4 (a preferential cyclase) disrupted gastrulation. Our data highlight the importance of ADP-ribosyl cyclases during embryogenesis.

SUBMITTER: Ramakrishnan L

PROVIDER: S-EPMC5435102 | biostudies-literature | 2016 Jun

REPOSITORIES: biostudies-literature

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ADP-ribosyl cyclases regulate early development of the sea urchin.

Ramakrishnan Latha L Uhlinger Kevin K Dale Leslie L Hamdoun Amro A Patel Sandip S

Messenger (Los Angeles, Calif. : Print) 20160601 1-2

ADP-ribosyl cyclases are multifunctional enzymes involved in the metabolism of nucleotide derivatives necessary for Ca2+ signalling such as cADPR and NAADP. Although Ca2+ signalling is a critical regulator of early development, little is known of the role of ADP-ribosyl cyclases during embryogenesis. Here we analyze the expression, activity and function of ADP-ribosyl cyclases in the embryo of the sea urchin - a key organism for study of both Ca2+ signalling and ...[more]

PMID: 28529830

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