Unknown

Dataset Information

0

Clonal expansion of genome-intact HIV-1 in functionally polarized Th1 CD4+ T cells.


ABSTRACT: HIV-1 causes a chronic, incurable disease due to its persistence in CD4+ T cells that contain replication-competent provirus, but exhibit little or no active viral gene expression and effectively resist combination antiretroviral therapy (cART). These latently infected T cells represent an extremely small proportion of all circulating CD4+ T cells but possess a remarkable long-term stability and typically persist throughout life, for reasons that are not fully understood. Here we performed massive single-genome, near-full-length next-generation sequencing of HIV-1 DNA derived from unfractionated peripheral blood mononuclear cells, ex vivo-isolated CD4+ T cells, and subsets of functionally polarized memory CD4+ T cells. This approach identified multiple sets of independent, near-full-length proviral sequences from cART-treated individuals that were completely identical, consistent with clonal expansion of CD4+ T cells harboring intact HIV-1. Intact, near-full-genome HIV-1 DNA sequences that were derived from such clonally expanded CD4+ T cells constituted 62% of all analyzed genome-intact sequences in memory CD4 T cells, were preferentially observed in Th1-polarized cells, were longitudinally detected over a duration of up to 5 years, and were fully replication- and infection-competent. Together, these data suggest that clonal proliferation of Th1-polarized CD4+ T cells encoding for intact HIV-1 represents a driving force for stabilizing the pool of latently infected CD4+ T cells.

SUBMITTER: Lee GQ 

PROVIDER: S-EPMC5490740 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

altmetric image

Publications


HIV-1 causes a chronic, incurable disease due to its persistence in CD4+ T cells that contain replication-competent provirus, but exhibit little or no active viral gene expression and effectively resist combination antiretroviral therapy (cART). These latently infected T cells represent an extremely small proportion of all circulating CD4+ T cells but possess a remarkable long-term stability and typically persist throughout life, for reasons that are not fully understood. Here we performed massi  ...[more]

Similar Datasets

| S-EPMC8722732 | biostudies-literature
| S-EPMC9338453 | biostudies-literature
| S-EPMC4645661 | biostudies-literature
| S-EPMC2851806 | biostudies-other
| S-EPMC4262401 | biostudies-literature
2016-07-11 | E-MTAB-4685 | biostudies-arrayexpress
| S-EPMC4975415 | biostudies-literature
| S-EPMC5687253 | biostudies-literature
| S-EPMC5960642 | biostudies-literature
| S-EPMC7136607 | biostudies-literature