Ontology highlight
ABSTRACT:
SUBMITTER: Glyde R
PROVIDER: S-EPMC5505868 | biostudies-literature | 2017 Jul
REPOSITORIES: biostudies-literature
Glyde Robert R Ye Fuzhou F Darbari Vidya Chandran VC Zhang Nan N Buck Martin M Zhang Xiaodong X
Molecular cell 20170601 1
Gene transcription is carried out by RNA polymerases (RNAPs). For transcription to occur, the closed promoter complex (RPc), where DNA is double stranded, must isomerize into an open promoter complex (RPo), where the DNA is melted out into a transcription bubble and the single-stranded template DNA is delivered to the RNAP active site. Using a bacterial RNAP containing the alternative σ<sup>54</sup> factor and cryoelectron microscopy, we determined structures of RPc and the activator-bound inter ...[more]