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The adipokine leptin modulates adventitial pericyte functions by autocrine and paracrine signalling.

ABSTRACT: Transplantation of adventitial pericytes (APCs) improves recovery from tissue ischemia in preclinical animal models by still unknown mechanisms. This study investigates the role of the adipokine leptin (LEP) in the regulation of human APC biological functions. Transcriptomic analysis of APCs showed components of the LEP signalling pathway are modulated by hypoxia. Kinetic studies indicate cultured APCs release high amounts of immunoreactive LEP following exposure to hypoxia, continuing upon return to normoxia. Secreted LEP activates an autocrine/paracrine loop through binding to the LEP receptor (LEPR) and induction of STAT3 phosphorylation. Titration studies using recombinant LEP and siRNA knockdown of LEP or LEPR demonstrate the adipokine exerts important regulatory roles in APC growth, survival, migration and promotion of endothelial network formation. Heterogeneity in LEP expression and secretion may influence the reparative proficiency of APC therapy. Accordingly, the levels of LEP secretion predict the microvascular outcome of APCs transplantation in a mouse limb ischemia model. Moreover, we found that the expression of the Lepr gene is upregulated on resident vascular cells from murine ischemic muscles, thus providing a permissive milieu to transplanted LEP-expressing APCs. Results highlight a new mechanism responsible for APC adaptation to hypoxia and instrumental to vascular repair.

SUBMITTER: Riu F

PROVIDER: S-EPMC5511138 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature

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The adipokine leptin modulates adventitial pericyte functions by autocrine and paracrine signalling.

Riu Federica F Slater Sadie C SC Garcia Eva Jover EJ Rodriguez-Arabaolaza Iker I Alvino Valeria V Avolio Elisa E Mangialardi Giuseppe G Cordaro Andrea A Satchell Simon S Zebele Carlo C Caporali Andrea A Angelini Gianni G Madeddu Paolo P

Scientific reports 20170714 1

Transplantation of adventitial pericytes (APCs) improves recovery from tissue ischemia in preclinical animal models by still unknown mechanisms. This study investigates the role of the adipokine leptin (LEP) in the regulation of human APC biological functions. Transcriptomic analysis of APCs showed components of the LEP signalling pathway are modulated by hypoxia. Kinetic studies indicate cultured APCs release high amounts of immunoreactive LEP following exposure to hypoxia, continuing upon retu ...[more]

PMID: 28710369

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The adipokine leptin modulates adventitial pericyte functions by autocrine and paracrine signalling.

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The adipokine leptin modulates adventitial pericyte functions by autocrine and paracrine signalling.

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