Ontology highlight
ABSTRACT:
SUBMITTER: Tao R
PROVIDER: S-EPMC5555402 | biostudies-literature | 2017 Jul
REPOSITORIES: biostudies-literature
Tao Rongkun R Zhao Yuzheng Y Chu Huanyu H Wang Aoxue A Zhu Jiahuan J Chen Xianjun X Zou Yejun Y Shi Mei M Liu Renmei R Su Ni N Du Jiulin J Zhou Hai-Meng HM Zhu Linyong L Qian Xuhong X Liu Haiyan H Loscalzo Joseph J Yang Yi Y
Nature methods 20170605 7
Reduced nicotinamide adenine dinucleotide phosphate (NADPH) is essential for biosynthetic reactions and antioxidant functions; however, detection of NADPH metabolism in living cells remains technically challenging. We develop and characterize ratiometric, pH-resistant, genetically encoded fluorescent indicators for NADPH (iNap sensors) with various affinities and wide dynamic range. iNap sensors enabled quantification of cytosolic and mitochondrial NADPH pools that are controlled by cytosolic NA ...[more]