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Utilizing Paper-Based Devices for Antimicrobial-Resistant Bacteria Detection.

ABSTRACT: Antimicrobial resistance (AMR), the ability of a bacterial species to resist the action of an antimicrobial drug, has been on the rise due to the widespread use of antimicrobial agents. Per the World Health Organization, AMR has an estimated annual cost of USD?34?billion in the US and is predicted to be the number one cause of death worldwide by 2050. One way AMR bacteria can spread, and by which individuals can contract AMR infections, is through contaminated water. Monitoring AMR bacteria in the environment currently requires that samples be transported to a central laboratory for slow and labor intensive tests. We have developed an inexpensive assay using paper-based analytical devices (PADs) that can test for the presence of ?-lactamase-mediated resistance. To demonstrate viability, the PAD was used to detect ?-lactam resistance in wastewater and sewage and identified resistance in individual bacterial species isolated from environmental water sources.

SUBMITTER: Boehle KE

PROVIDER: S-EPMC5568866 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature

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Utilizing Paper-Based Devices for Antimicrobial-Resistant Bacteria Detection.

Boehle Katherine E KE Gilliand Jake J Wheeldon Christopher R CR Holder Amethyst A Adkins Jaclyn A JA Geiss Brian J BJ Ryan Elizabeth P EP Henry Charles S CS

Angewandte Chemie (International ed. in English) 20170505 24

Antimicrobial resistance (AMR), the ability of a bacterial species to resist the action of an antimicrobial drug, has been on the rise due to the widespread use of antimicrobial agents. Per the World Health Organization, AMR has an estimated annual cost of USD 34 billion in the US and is predicted to be the number one cause of death worldwide by 2050. One way AMR bacteria can spread, and by which individuals can contract AMR infections, is through contaminated water. Monitoring AMR bacteria in t ...[more]

PMID: 28474847

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Project description:Numerous fabrication methods have been reported for microfluidic paper-based analytical devices (?PADs) using barrier materials ranging from photoresist to wax. While these methods have been used with wide success, consistently producing small, high-resolution features using materials and methods that are compatible with solvents and surfactants remains a challenge. Two new methods are presented here for generating ?PADs with well-defined, high-resolution structures compatible with solvents and surfactant-containing solutions by partially or fully fusing paper with Parafilm® followed by cutting with a CO2 laser cutter. Partial fusion leads to laminated paper (l-paper) while the complete fusion results in infused paper (i-paper). Patterned structures in l-paper were fabricated by selective removal of the paper but not the underlying Parafilm® using a benchtop CO2 laser. Under optimized conditions, a gap as small as 137 ± 22 ?m could be generated. Using this approach, a miniaturized paper 384-zone plate, consisting of circular detection elements with a diameter of 1.86 mm, was fabricated in 64 × 43 mm2 area. Furthermore, these ablation-patterned substrates were confirmed to be compatible with surfactant solutions and common organic solvents (methanol, acetonitrile and dimethylformamide), which has been achieved by very few ?PAD patterning techniques. Patterns in i-paper were created by completely cutting out zones of the i-paper and then fixing pre-cut paper into these openings similar to the strategy of fitting a jigsaw piece into a puzzle. Upon heating, unmodified paper was readily sealed into these openings due to partial reflow of the paraffin into the paper. This unique and simple bonding method was illustrated by two types of 3D ?PADs, a push-on valve and a time-gated flow distributor, without adding adhesive layers. The free-standing jigsaw-patterned sheets showed good structural stability and solution compatibility, which provided a facile alternative method for fabricating complicated ?PADs.

Dataset Information

Utilizing Paper-Based Devices for Antimicrobial-Resistant Bacteria Detection.

Publications

Utilizing Paper-Based Devices for Antimicrobial-Resistant Bacteria Detection.

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