Dataset Information

Computed exercise plasma lactate concentrations: A conversion formula.

ABSTRACT:

Objectives

Blood lactate measurements are common as a marker of skeletal muscle metabolism in sport medicine. Due to the close equilibrium between the extracellular and intramyocellular space, plasma lactate is a more accurate estimate of muscle lactate. However, whole blood-based lactate measurements are more convenient in field use. The purpose of this investigation was therefore (1) to establish a plasma-converting lactate formula for field use, and (2) to validate the computed plasma lactate levels by comparison to a laboratory standard method.

Design and methods

A total of 91 venous samples were taken from 6 individuals with type 1 diabetes during resting and exercise conditions and assessed for whole blood and plasma lactate using the YSI 2300 analyzer. A linear model was applied to establish a formula for converting whole blood lactate to plasma lactate. The validity of computed plasma lactate values was assessed by comparison to a laboratory standard method.

Results

Whole blood YSI lactate could be converted to plasma YSI values (slope 1.66, intercept 0.12) for samples with normal hematocrit. Computed plasma levels compared to values determined by the laboratory standard method using Passing-Bablok regression yielded a slope of 1.03 (95%CI:0.99:1.08) with an intercept of -0.11 (95%CI:-0.18:-0.06).

Conclusions

Whole blood YSI lactate values can be reliably converted into plasma values which are in line with laboratory determined plasma measurements.

SUBMITTER: Bally L

PROVIDER: S-EPMC5574503 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature

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Publications

Computed exercise plasma lactate concentrations: A conversion formula.

Bally Lia L Zueger Thomas T Stettler Christoph C Leichtle Alexander Benedikt AB

Practical laboratory medicine 20151128

<h4>Objectives</h4>Blood lactate measurements are common as a marker of skeletal muscle metabolism in sport medicine. Due to the close equilibrium between the extracellular and intramyocellular space, plasma lactate is a more accurate estimate of muscle lactate. However, whole blood-based lactate measurements are more convenient in field use. The purpose of this investigation was therefore (1) to establish a plasma-converting lactate formula for field use, and (2) to validate the computed plasma ...[more]

PMID: 28856187

Similar Datasets

Project description:BackgroundBranched-chain amino acids (BCAA) i.e., leucine (Leu), isoleucine (Ile) and valine (Val) are important amino acids, which metabolism play a role in maintaining system energy homeostasis at rest and during exercise. As recently shown lowering of circulating BCAA level improves insulin sensitivity and cardiac metabolic health. However, little is known concerning the impact of a single bout of incremental exercise and physical training on the changes in blood BCAA. The present study aimed to determine the impact of a gradually increasing exercise intensity-up to maximal oxygen uptake (VO2max) on the changes of the plasma BCAA [?BCAA]pl, before and after 5-weeks of moderate-intensity endurance training (ET).MethodsTen healthy young, untrained men performed an incremental cycling exercise test up to exhaustion to reach VO2max, before and after ET.ResultsWe have found that exercise of low-to-moderate intensity (up to ?50% of VO2max lasting about 12 min) had no significant effect on the [?BCAA]pl, however the exercise of higher intensity (above 70% of VO2max lasting about 10 min) resulted in a pronounced decrease (p < 0.05) in [?BCAA]pl. The lowering of plasma BCAA when performing exercise of higher intensity was preceded by a significant increase in plasma lactate concentration, showing that a significant attenuation of BCAA during incremental exercise coincides with exercise-induced acceleration of glycogen utilization. In addition, endurance training, which significantly increased power generating capabilities at VO2max (p = 0.004) had no significant impact on the changes of [?BCAA]pl during this incremental exercise.ConclusionWe have concluded that an exercise of moderate intensity of relatively short duration generally has no effect on the [?BCAA]pl in young, healthy men, whereas significant decrease in [?BCAA]pl occurs when performing exercise in heavy-intensity domain. The impact of exercise intensity on the plasma BCAA concentration seems to be especially important for patients with cardiometabolic risk undertaken cardiac rehabilitation or recreational activity.

Dataset Information

Computed exercise plasma lactate concentrations: A conversion formula.

Objectives

Design and methods

Results

Conclusions

Publications

Computed exercise plasma lactate concentrations: A conversion formula.

Similar Datasets

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