Ontology highlight
ABSTRACT:
SUBMITTER: Parish T
PROVIDER: S-EPMC56589 | biostudies-literature | 2001
REPOSITORIES: biostudies-literature
BMC microbiology 20010831
<h4>Background</h4>The acetamidase of Mycobacterium smegmatis is a highly inducible enzyme. Expression of this enzyme is increased 100-fold when the substrate acetamide is present. The acetamidase gene is found immediately downstream of three open reading frames. Two of these are proposed to be involved in regulation.<h4>Results</h4>We constructed a deletion mutant in one of the upstream ORFs (amiA). This mutant (Mad1) showed a constitutively high level of acetamidase expression. We identified f ...[more]