Dataset Information

Microbial agents in macroscopically healthy mammary gland tissues of small ruminants.

ABSTRACT:

Background

Health of mammary glands is fundamental for milk and dairy products hygiene and quality, with huge impacts on consumers welfare.

Methods

This study aims to investigate the microbial agents (bacteria, fungi and lentiviruses) isolated from 89 macroscopically healthy udders of regularly slaughtered small ruminants (41 sheep, 48 goats), also correlating their presence with the histological findings. Multinomial logistic regression was applied to evaluate the association between lesions and positivity for different microbial isolates, animal age and bacteria.

Results

Twenty-five samples were microbiologically negative; 138 different bacteria were isolated in 64 positive udders. Coagulase-negative staphylococci were the most prevalent bacteria isolated (46.42%), followed by environmental opportunists (34.76%), others (10.14%) and pathogens (8.68%). Most mammary glands showed coinfections (75%). Lentiviruses were detected in 39.3% of samples. Histologically, chronic non-suppurative mastitis was observed in 45/89 glands, followed by chronic mixed mastitis (12/89) and acute suppurative mastitis (4/89). Only 28 udders were normal. Histological lesions were significantly associated with the animal species and lentiviruses and coagulase-negative staphylococci infections. Goats had significantly higher risk to show chronic mixed mastitis compared to sheep. Goats showed a significantly lower risk (OR = 0.26; 95% CI [0.06-0.71]) of being infected by environmental opportunists compared to sheep, but higher risk (OR = 10.87; 95% CI [3.69-37.77]) of being infected with lentiviruses.

Discussion

The results of the present study suggest that macroscopically healthy glands of small ruminants could act as a reservoir of microbial agents for susceptible animals, representing a potential risk factor for the widespread of acute or chronic infection in the flock.

SUBMITTER: Spuria L

PROVIDER: S-EPMC5689019 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature

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Publications

Microbial agents in macroscopically healthy mammary gland tissues of small ruminants.

Spuria Liliana L Biasibetti Elena E Bisanzio Donal D Biasato Ilaria I De Meneghi Daniele D Nebbia Patrizia P Robino Patrizia P Bianco Paolo P Lamberti Michele M Caruso Claudio C Di Blasio Alessia A Peletto Simone S Masoero Loretta L Dondo Alessandro A Capucchio Maria Teresa MT

PeerJ 20171113

<h4>Background</h4>Health of mammary glands is fundamental for milk and dairy products hygiene and quality, with huge impacts on consumers welfare.<h4>Methods</h4>This study aims to investigate the microbial agents (bacteria, fungi and lentiviruses) isolated from 89 macroscopically healthy udders of regularly slaughtered small ruminants (41 sheep, 48 goats), also correlating their presence with the histological findings. Multinomial logistic regression was applied to evaluate the association bet ...[more]

PMID: 29152416

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Project description:BackgroundMicroRNAs (miRNAs) have a great influence on various physiological functions. A lot of high-throughput sequencing (HTS) research on miRNAs has been executed in the caprine mammary gland at different lactation periods (common milk lactation and dry period), but little is known about differentially expressed miRNAs in the caprine mammary gland of colostrum and peak lactation periods.ResultThis study identified 131 differentially expressed miRNAs (P?<?0.05 and log2 colostrum normalized expression (NE)/peak lactation NE?>?1 or log2 colostrum NE/peak lactation NE?<?-1), including 57 known miRNAs and 74 potential novel miRNAs in the colostrum and peak lactation libraries. In addition, compared with differentially expressed miRNAs in the peak lactation period, 45 miRNAs in the colostrum lactation period were remarkably upregulated, whereas 86 miRNAs were markedly downregulated (P?<?0.05 and log2 colostrum NE/peak lactation NE?>?1 or log2 colostrum NE/peak lactation NE?<?-1). The expressions of 10 randomly selected miRNAs was analyzed through stem-loop real-time quantitative PCR (RT-qPCR). Their expression patterns were the same with Solexa sequencing results. Pathway analysis suggested that oestrogen, endocrine, adipocytokine, oxytocin and MAPK signalling pathways act on the development of mammary gland and milk secretion importantly. In addition, the miRNA-target-network showed that the bta-miR-574 could influence the development of mammary gland and lactation by leptin receptor (LEPR), which was in the adipocytokine signalling pathway. Chr5_3880_mature regulated mammary gland development and lactation through Serine/threonine-protein phosphatase (PPP1CA), which was in the oxytocin signalling pathway.ConclusionsOur finding suggested that the profiles of miRNAs were related to the physiological functions of mammary gland in the colostrum and peak lactation periods. The biological features of these miRNAs may help to clarify the molecular mechanisms of lactation and the development of caprine mammary gland.

Dataset Information

Microbial agents in macroscopically healthy mammary gland tissues of small ruminants.

Background

Methods

Results

Discussion

Publications

Microbial agents in macroscopically healthy mammary gland tissues of small ruminants.

Similar Datasets

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