Ontology highlight
ABSTRACT:
SUBMITTER: Lalit PA
PROVIDER: S-EPMC5693216 | biostudies-literature | 2017 May
REPOSITORIES: biostudies-literature
Lalit Pratik A PA Rodriguez Adriana M AM Downs Karen M KM Kamp Timothy J TJ
Nature protocols 20170420 5
Here we describe a protocol to generate expandable and multipotent induced cardiac progenitor cells (iCPCs) from mouse adult fibroblasts using forced expression of Mesp1, Tbx5, Gata4, Nkx2.5 and Baf60c (MTGNB) along with activation of Wnt and JAK/STAT signaling. This method does not use iPS cell factors and thus differs from cell activation and signaling-directed (CASD) reprogramming to cardiac progenitors. Our method is specific to direct CPC reprogramming, whereas CASD reprogramming can genera ...[more]