Ontology highlight
ABSTRACT:
SUBMITTER: Vanle BC
PROVIDER: S-EPMC5746426 | biostudies-literature | 2017 Oct
REPOSITORIES: biostudies-literature
Vanle Brigitte C BC Florang Virginia R VR Murry Daryl J DJ Aguirre Arturo L AL Doorn Jonathan A JA
Biochemical and biophysical research communications 20170819 2
<h4>Background</h4>The aldehyde metabolite of dopamine, 3,4-dihydroxyphenylacetaldehyde (DOPAL) is an endogenous neurotoxin implicated in Parkinson's Disease. Elucidating protein targets of DOPAL is essential in understanding it's pathology. The enzyme, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a target of DOPAL.<h4>Methods</h4>GAPDH activity was measured via reduction of NAD<sup>+</sup> cofactor (340 nm). Protein aggregation was assessed with SDS-PAGE methods and specific modification ...[more]