Dissemination of Multidrug-Resistant Proteus mirabilis Clones Carrying a Novel Integron-Borne blaIMP-1 in a Tertiary Hospital.
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ABSTRACT:
SUBMITTER: Ramos AC
PROVIDER: S-EPMC5786801 | biostudies-literature | 2018 Feb
REPOSITORIES: biostudies-literature
Publications
Dissemination of Multidrug-Resistant Proteus mirabilis Clones Carrying a Novel Integron-Borne <i>bla</i><sub>IMP-1</sub> in a Tertiary Hospital.
Ramos Ana Carolina AC Cayô Rodrigo R Carvalhaes Cecilia Godoy CG Jové Thomas T da Silva Greice Pereira GP Sancho Fernanda Maciel Paschoin FMP Chagas-Neto Thomas T Medeiros Eduardo Alexandrino Servolo EAS Gales Ana Cristina AC
Antimicrobial agents and chemotherapy 20180125 2
This study aimed to characterize multidrug-resistant <i>Proteus mirabilis</i> clones carrying a novel class 1 integron-borne <i>bla</i><sub>IMP-1</sub> In<i>1359</i> was inserted into a large conjugative plasmid that also carried <i>bla</i><sub>CTX-M-2</sub> The production of carbapenemases in <i>Enterobacteriaceae</i> that are intrinsically resistant to polymyxins and tigecycline is very worrisome, representing a serious challenge to clinicians and infection control teams. ...[more]
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Project description:A plasmid-mediated metallo-beta-lactamase gene was cloned from a carbapenem-resistant Serratia marcescens strain, AK9373. The metallo-beta-lactamase gene was identical to the blaIMP, and it was located in the space between an integrase-like gene and an aac(6')-Ib-like gene. The deduced amino acid sequence for the putative integrase gene showed considerable identity (60.9%) to that of the Escherichia coli integrase reported. Sequences similar to the GTTRRRY and an atypical 59-base element containing a 67-bp inverted repeat sequence, which were peculiar to the integrase-dependent recombination, were also conserved in the flanking regions of the blaIMP gene. These findings imply that the metallo-beta-lactamase gene in S. marcescens AK9373 is carried by a novel integron-like element that is mediated by a transferable large plasmid.
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