Ontology highlight
ABSTRACT:
SUBMITTER: Huang TH
PROVIDER: S-EPMC5843376 | biostudies-literature | 2018 Mar
REPOSITORIES: biostudies-literature
Huang Ting-Hsiang TH Fowler Faith F Chen Chin-Chuan CC Shen Zih-Jie ZJ Sleckman Barry B Tyler Jessica K JK
Molecular cell 20180222 5
The access-repair-restore model for the role of chromatin in DNA repair infers that chromatin is a mere obstacle to DNA repair. However, here we show that blocking chromatin assembly, via knockdown of the histone chaperones ASF1 or CAF-1 or a mutation that prevents ASF1A binding to histones, hinders Rad51 loading onto ssDNA during homologous recombination. This is a consequence of reduced recruitment of the Rad51 loader MMS22L-TONSL to ssDNA, resulting in persistent RPA foci, extensive DNA end r ...[more]