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Commonly used trafficking blocks disrupt ARF1 activation and the localization and function of specific Golgi proteins.


ABSTRACT: Cold temperature blocks used to synchronize protein trafficking inhibit GBF1 function, leading to a decrease in ARF1-GTP levels and mislocalization of the ARF1 effector golgin-160. Several other, but not all, Golgi proteins including ARL1 also mislocalize. ARF1 activity and golgin-160 localization require more than 30 min to recover from these blocks.

SUBMITTER: Gilbert CE 

PROVIDER: S-EPMC5896932 | biostudies-literature |

REPOSITORIES: biostudies-literature

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