Commonly used trafficking blocks disrupt ARF1 activation and the localization and function of specific Golgi proteins.
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ABSTRACT: Cold temperature blocks used to synchronize protein trafficking inhibit GBF1 function, leading to a decrease in ARF1-GTP levels and mislocalization of the ARF1 effector golgin-160. Several other, but not all, Golgi proteins including ARL1 also mislocalize. ARF1 activity and golgin-160 localization require more than 30 min to recover from these blocks.
SUBMITTER: Gilbert CE
PROVIDER: S-EPMC5896932 | biostudies-literature |
REPOSITORIES: biostudies-literature
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