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Identification of multiple proteoforms biomarkers on clinical samples by routine Top-Down approaches.

ABSTRACT: Top-Down approaches have an extremely high biological relevance, especially when it comes to biomarker discovery, but the necessary pre-fractionation constraints are not easily compatible with the robustness requirements and the size of clinical sample cohorts. We have demonstrated that intact protein profiling studies could be run on UHR-Q-ToF with limited pre-fractionation (Schmit et al., 2017) [1]. The dataset presented herein is an extension of this research. Proteoforms known to play a role in the pathophysiology process of Alzheimer's disease were identified as candidate biomarkers. In this article, mass spectrometry performance of these candidates are demonstrated.

SUBMITTER: Vialaret J

PROVIDER: S-EPMC5996497 | biostudies-literature | 2018 Jun

REPOSITORIES: biostudies-literature

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Identification of multiple proteoforms biomarkers on clinical samples by routine Top-Down approaches.

Vialaret Jerome J Schmit Pierre-Olivier PO Lehmann Sylvain S Gabelle Audrey A Wood Jason J Bern Marshall M Paape Rainer R Suckau Detlev D Kruppa Gary G Hirtz Christophe C

Data in brief 20180331

Top-Down approaches have an extremely high biological relevance, especially when it comes to biomarker discovery, but the necessary pre-fractionation constraints are not easily compatible with the robustness requirements and the size of clinical sample cohorts. We have demonstrated that intact protein profiling studies could be run on UHR-Q-ToF with limited pre-fractionation (Schmit et al., 2017) [1]. The dataset presented herein is an extension of this research. Proteoforms known to play a role ...[more]

PMID: 29900270

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