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Preferential Mitochondrial Localization of a Goniothalamin Fluorescent Derivative.

ABSTRACT: A fluorescent 2,1,3-benzothiadiazole-containing goniothalamin derivative, BTD-GTN (1), has been synthesized and successfully tested in bioimaging experiments in live cells. The fluorescent compound proved to be capable of transposing the cell membranes, indicating its subcellular localization. The use of the benzothiadiazole core as the fluorophore revealed the favored localization of the GTN analogue 1 in the cytoplasm of live cells, preferentially in the mitochondria, in line with previous results that indicated the loss of mitochondrial transmembrane potential upon treatment with GTN. The results described herein highlight the potential of the BTD-GTN hybrid structures for future studies regarding the cellular mechanism of action of this family of compounds.

SUBMITTER: Raitz I

PROVIDER: S-EPMC6044949 | biostudies-literature | 2017 Jul

REPOSITORIES: biostudies-literature

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Preferential Mitochondrial Localization of a Goniothalamin Fluorescent Derivative.

Raitz Ismael I de Souza Filho Roberto Y RY de Andrade Lorena P LP Correa Jose R JR Neto Brenno A D BAD Pilli Ronaldo A RA

ACS omega 20170720 7

A fluorescent 2,1,3-benzothiadiazole-containing goniothalamin derivative, BTD-GTN (<b>1</b>), has been synthesized and successfully tested in bioimaging experiments in live cells. The fluorescent compound proved to be capable of transposing the cell membranes, indicating its subcellular localization. The use of the benzothiadiazole core as the fluorophore revealed the favored localization of the GTN analogue <b>1</b> in the cytoplasm of live cells, preferentially in the mitochondria, in line wit ...[more]

PMID: 30023703

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