PLK1- and PLK4-Mediated Asymmetric Mitotic Centrosome Size and Positioning in the Early Zebrafish Embryo.

ABSTRACT: Factors that regulate mitotic spindle positioning remain unclear within the confines of extremely large embryonic cells, such as the early divisions of the vertebrate embryo, Danio rerio (zebrafish). We find that the mitotic centrosome, a structure that assembles the mitotic spindle [1], is notably large in the zebrafish embryo (246.44 ± 11.93 μm² in a 126.86 ± 0.35 μm diameter cell) compared to a C. elegans embryo (5.78 ± 0.18 μm² in a 55.83 ± 1.04 μm diameter cell). During embryonic cell divisions, cell size changes rapidly in both C. elegans and zebrafish [2, 3], where mitotic centrosome area scales more closely with changes in cell size compared to changes in spindle length. Embryonic zebrafish spindles contain asymmetrically sized mitotic centrosomes (2.14 ± 0.13

SUBMITTER: Rathbun LI 

PROVIDER: S-EPMC8159022 | biostudies-literature | 2020 Nov

REPOSITORIES: biostudies-literature