Unknown

Dataset Information

0

Graphene oxide-based biosensing platform for rapid and sensitive detection of HIV-1 protease.


ABSTRACT: HIV-1 protease is essential for the life cycle of the human immunodeficiency virus (HIV), and is one of the most important clinical targets for antiretroviral therapies. In this work, we developed a graphene oxide (GO)-based fluorescence biosensing platform for the rapid, sensitive, and accurate detection of HIV-1 protease, in which fluorescent-labeled HIV-1 protease substrate peptide molecules were covalently linked to GO. In the absence of HIV-1 protease, fluorescein was effectively quenched by GO. In contrast, in the presence of HIV-1 protease, it would cleave the substrate peptide into short fragments, thus producing fluorescence. Based on this sensing strategy, HIV-1 protease could be detected at as low as 1.18 ng/mL. More importantly, the sensor could successfully detect HIV-1 protease in human serum. Such GO-based fluorescent sensors may find useful applications in many fields, including diagnosis of protease-related diseases, as well as sensitive and high-throughput screening of drug candidates. Graphical abstract ?.

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC6159923 | biostudies-literature | 2018 Sep

REPOSITORIES: biostudies-literature

altmetric image

Publications

Graphene oxide-based biosensing platform for rapid and sensitive detection of HIV-1 protease.

Zhang Youwen Y   Chen Xiaohan X   Roozbahani Golbarg M GM   Guan Xiyun X  

Analytical and bioanalytical chemistry 20180702 24


HIV-1 protease is essential for the life cycle of the human immunodeficiency virus (HIV), and is one of the most important clinical targets for antiretroviral therapies. In this work, we developed a graphene oxide (GO)-based fluorescence biosensing platform for the rapid, sensitive, and accurate detection of HIV-1 protease, in which fluorescent-labeled HIV-1 protease substrate peptide molecules were covalently linked to GO. In the absence of HIV-1 protease, fluorescein was effectively quenched b  ...[more]

Similar Datasets

| S-EPMC6800036 | biostudies-literature
| S-EPMC5507977 | biostudies-literature
| S-EPMC7085571 | biostudies-literature
| S-EPMC4701278 | biostudies-literature
| S-EPMC6480299 | biostudies-literature
| S-EPMC10335395 | biostudies-literature
| S-EPMC6694116 | biostudies-literature
| S-EPMC8470607 | biostudies-literature
| S-EPMC5215691 | biostudies-literature
| S-EPMC7763624 | biostudies-literature