Dataset Information

Using sound pulses to solve the crystal-harvesting bottleneck.

ABSTRACT: Crystal harvesting has proven to be difficult to automate and remains the rate-limiting step for many structure-determination and high-throughput screening projects. This has resulted in crystals being prepared more rapidly than they can be harvested for X-ray data collection. Fourth-generation synchrotrons will support extraordinarily rapid rates of data acquisition, putting further pressure on the crystal-harvesting bottleneck. Here, a simple solution is reported in which crystals can be acoustically harvested from slightly modified MiTeGen In Situ-1 crystallization plates. This technique uses an acoustic pulse to eject each crystal out of its crystallization well, through a short air column and onto a micro-mesh (improving on previous work, which required separately grown crystals to be transferred before harvesting). Crystals can be individually harvested or can be serially combined with a chemical library such as a fragment library.

SUBMITTER: Samara YN

PROVIDER: S-EPMC6173054 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature

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Using sound pulses to solve the crystal-harvesting bottleneck.

Samara Yasmin N YN Brennan Haley M HM McCarthy Liam L Bollard Mary T MT Laspina Denise D Wlodek Jakub M JM Campos Stefanie L SL Natarajan Ramya R Gofron Kazimierz K McSweeney Sean S Soares Alexei S AS Leroy Ludmila L

Acta crystallographica. Section D, Structural biology 20181002 Pt 10

Crystal harvesting has proven to be difficult to automate and remains the rate-limiting step for many structure-determination and high-throughput screening projects. This has resulted in crystals being prepared more rapidly than they can be harvested for X-ray data collection. Fourth-generation synchrotrons will support extraordinarily rapid rates of data acquisition, putting further pressure on the crystal-harvesting bottleneck. Here, a simple solution is reported in which crystals can be acous ...[more]

PMID: 30289409

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Dataset Information

Using sound pulses to solve the crystal-harvesting bottleneck.

Publications

Using sound pulses to solve the crystal-harvesting bottleneck.

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