Unknown

Dataset Information

0

REX1 is the critical target of RNF12 in imprinted X chromosome inactivation in mice.

ABSTRACT: In mice, imprinted X chromosome inactivation (iXCI) of the paternal X in the pre-implantation embryo and extraembryonic tissues is followed by X reactivation in the inner cell mass (ICM) of the blastocyst to facilitate initiation of random XCI (rXCI) in all embryonic tissues. RNF12 is an E3 ubiquitin ligase that plays a key role in XCI. RNF12 targets pluripotency protein REX1 for degradation to initiate rXCI in embryonic stem cells (ESCs) and loss of the maternal copy of Rnf12 leads to embryonic lethality due to iXCI failure. Here, we show that loss of Rex1 rescues the rXCI phenotype observed in Rnf12-/- ESCs, and that REX1 is the prime target of RNF12 in ESCs. Genetic ablation of Rex1 in Rnf12-/- mice rescues the Rnf12-/- iXCI phenotype, and results in viable and fertile Rnf12-/-:Rex1-/- female mice displaying normal iXCI and rXCI. Our results show that REX1 is the critical target of RNF12 in XCI.

SUBMITTER: Gontan C 

PROVIDER: S-EPMC6232137 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature

Json Xml
altmetric image

Publications

REX1 is the critical target of RNF12 in imprinted X chromosome inactivation in mice.

Gontan Cristina C   Mira-Bontenbal Hegias H   Magaraki Aristea A   Dupont Catherine C   Barakat Tahsin Stefan TS   Rentmeester Eveline E   Demmers Jeroen J   Gribnau Joost J  

Nature communications 20181112 1

In mice, imprinted X chromosome inactivation (iXCI) of the paternal X in the pre-implantation embryo and extraembryonic tissues is followed by X reactivation in the inner cell mass (ICM) of the blastocyst to facilitate initiation of random XCI (rXCI) in all embryonic tissues. RNF12 is an E3 ubiquitin ligase that plays a key role in XCI. RNF12 targets pluripotency protein REX1 for degradation to initiate rXCI in embryonic stem cells (ESCs) and loss of the maternal copy of Rnf12 leads to embryonic  ...[more]

Similar Datasets

Proteomics REX1 is the critical target of RNF12 in imprinted X chromosome inactivation in mice.
2018-11-15 | PXD010944 | Pride
Unknown Rlim/Rnf12, Rex1, and X Chromosome Inactivation.
| S-EPMC6834644 | biostudies-literature
Unknown Maternal Rnf12/RLIM is required for imprinted X-chromosome inactivation in mice.
| S-EPMC2967734 | biostudies-literature
Unknown Dynamic changes in paternal X-chromosome activity during imprinted X-chromosome inactivation in mice.
| S-EPMC2653561 | biostudies-literature
Unknown RNF12 activates Xist and is essential for X chromosome inactivation.
| S-EPMC3029249 | biostudies-other
Unknown The role of maternal-specific H3K9me3 modification in establishing imprinted X-chromosome inactivation and embryogenesis in mice.
| S-EPMC4243243 | biostudies-literature
Methylation profiling Maternal SMCHD1 controls both imprinted Xist expression and imprinted X chromosome inactivation
2021-10-24 | GSE186315 | GEO
Unknown Evidence for local regulatory control of escape from imprinted X chromosome inactivation.
| S-EPMC4063926 | biostudies-literature
Unknown Evidence of Xist RNA-independent initiation of mouse imprinted X-chromosome inactivation.
| S-EPMC2754729 | biostudies-literature
Unknown Ftx is dispensable for imprinted X-chromosome inactivation in preimplantation mouse embryos.
| S-EPMC5381492 | biostudies-literature